CellDETACHTM culture surface Products

Thank you for using a product with temperature-responsive JET CellDETACHTM culture surface. The temperature-sensitive polymer with thickness of 15-20 nanometer chemically covalently immobilized on the culture surface. Cells can be spontaneously intactly detached from JET CellDETACHTM culture surface under 20-25°C without enzymatic processing which is well known for its disfunction of cells. Keeping their activities is possible with JET CellDETACHTM culture surface for cell culture.

Applications

Ideal product for intactly harvesting cells, especially for fragile cells such as stem cells and neuron cells, and macrophage cells etc., and also for tissue culture, extra cellular matrix research, and so on. 

 

Cautions

Please ensure that there is no damage to the package and read the following instructions carefully to ensure the maximum performance of this product.
The product is intended for research purposes only. Any other use is not warranted by Jet coporation. Do not use the product for clinical or diagnostic purposes. Products with JET CellDETACHTM surface are intended for single-use only.

 

Disposal

Ensure the used product without biological and chemical pollution to the environment and disposed as recyclable plastics.
The product is prepared and shipped under high quality control. However, should you have any claims or questions, please contact us for free.

Figure1.(a)Schematic representaion of the adhesion(37 °C)and detachment(20°C)of a cell on a poly(N-isopropylamide)-grafted surface. Cell on PNIPAM-coated dishes at 37°C and 20°C. Note the elongated shape of cells on the left micrograph characteristic of a supported cell cultur, and rounded cell shape on the right characterstic.

 

Do not re-use the product as cell secreted and medium-derived components may be adsorbed to the surface after cell harvest, compromising performance in repeated use.

Re-sterilization and scratching of the culture surface may compromise the JET CellDETACHTM surface properties.

 

In vitro cell culture

The temperature-responsive polymer chemically covalently grafted to the culture surface area becomes slightly hydrophilic above 32°C, allowing cells to adhere. So it is recommended that cells are preferable incubated at 37°C.The JET CellDETACHTM surface becomes hydrophobic below 32°C, causing cell detachment from the surface.
To avoid undesired cell detachment during seeding cells and medium changes, pre-warming culture surface and culture medium (37°C) to facilitate cell attachment is suggested. Observations under a microscope should also be kept short in order to prevent cooling and undesired cell detachment. Seed cells evenly over the culture area to avoid uneven distribution of the cells in culture.
Attachment time for cells may vary with cell type.
Should you have difficulty in attaching cells, pre-coating of the culture surface with collagen, fibronectin, polylysine, laminin or the use of medium with serum may ease cell attachment.

 

Cells harvest
Culture cells on the JET CellDETACHTM surface to a density before confluence, that is cell-cell junctions are not established, cells harvest are readily conducted by slight agitation and pipetting.
Place the product with JET CellDETACHTM  surface in an environment set at 20°C in order to detach the cells. Alternatively, the product may be placed at room temperature (20–25°C) under sterile conditions. For quicker cell detachment, the culture medium can be exchanged to 20–25°C culture medium prior to detachment.
Cells should detach spontaneously within 20-40 minutes, some cells may not detach completely, depending on the cell type and detachment conditions.
Cells that fail to detach spontaneously can be detached by gentle plate/dish agitation, or by gently flushing the surface with medium.

 

Storage

The product should be stored at room temperature (20–35°C) and out of direct sunlight. 

CellDETACH TM  Culture Plate

Cat. No.	Well Qty.	Surface Type	Sterile	Cell Growth Area(cm2)	Qty.Per bag/case
CDP022006	6	CellDETACHTMSurface Treated	Y		1/100
CDP022012	12		Y		1/100
CDP022024	24		Y		1/100

CellDETACH TM  Culture  Dish 

Cat. No.	Diameter(mm)	Surface Type	Sterile	Cell Growth Area(cm2)	Qty.Per bag/case
CDD022035	35	CellDETACHTMSurface Treated	Y	8.5	10/960
CDD022060	60		Y	21.2	10/600
CDD022070	70		Y	36.3	10/600
CDD022090	90		Y	58.4	10/500
CDD022100	100		Y	60.8	10/300
CDD022150	150		Y	143.0	1/120

CellDETACH TM  Culture Flask 

Cat. No.	Volume(mm)	Surface Type	Sterile	Cell Growth Area(cm2)	Qty.Per bag/case
CDF022025	25	CellDETACHTMSurface Treated	Y	12.5	10/200
CDF022050	50		Y	25.0	10/200
CDF022250	250		Y	75.0	5/100
CDF022600	600		Y	182.0	5/40
CDF022850	850		Y	300.0	3/18