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CellDETACH™ Temperature-Responsive Cell Culture SurfaceUsing trypsin digestion or cell scraper to separate adherent cells will affect the expression of cell surface proteins, damage cell health and reduce cell viability. CellDETACH™ Temperature-Responsive cell culture surface is coated by an even and unique nano-polymer. When the temperature of the culture is reduced from 37℃to4℃, the temperature-responsive surface gradually changes from slightly hydrofobic to hydrophilic, resulting in adherent cells harvest without trypsin involved. By using this gently method, avoid cell damaging by scraper or trysin to preserve high viability and intact surface receptors and antigens of cells, to realize cell harvest without damage and continuous subculture procedure.
No trypsin, no scraper, temperature-induced harvesting of adherent cells. CellDETACH™ Temperature-Responsive cell culture surface is specially designed by the R&D team of JET BIOFIL for cell passage, cell transplantation, and has obtained a national invention patent (patent number: ZL201510780506.3), which aims to help experimenters to harvest single-cell cells sheet, build 3D tissue models linked by normal cells and extra cellular matrix, simplify cell culture and tissue engineering techniques, and minimize experimental manipulation time. l National invention patent (Patent Number: ZL201510780506.3) l Induce cell shedding simply by lowering the temperature, simple, fast and easy to operate l l No trypsin: preserve the intact cell surface proteins and markers l No cell scraping: avoid mechanical damage to cells and ensure high cell viability l Optimized cell culture and tissue engineering techniques
Scope of Application The Temperature-Responsive cell culture surface is suitable for the in vitro culture of most adherent cells, such as stem cells, neural cells, macrophages, cancer cells, etc. It is ideal for harmless cell harvesting. It can be widely used in expanded cell culture, cell therapy, 3D tissue modeling, extra cellular matrix research and other fields.
Instructions for Use In Vitro Cell Culture 1.When the temperature is above 32℃, the Temperature-Responsive polymer coating on the surface of CellDETACH TM Temperature-Responsive cell culture is in a polymer curl state, showing slight hydrophobicity. Facilitates cell attachment and growth. 2.When the temperature is below 32°C, the Temperature-Responsive polymer coating on the surface is in a polymer stretched state, which will bind water molecules and expand, showing hydrophilicity, which will facilitate the shedding of adherent cells. When the temperature drops to 4 °C, the shedding efficiency is the best. 3.When the temperature of the Temperature-Responsive cell culture surface drops below 32°C, excessive disturbance may cause cells to fall off, so please do not delay too much when taking pictures and observing during cell culture.
Cell Harvest v The best harvest is achieved when the confluence of cells is higher than 80%. v When harvesting cells, the culture environment temperature can be reduced to 4 ℃ , or the Temperature-Responsive products should be placed in a sterile incubator at 4 ℃, or the culture can be replaced with a culture medium of 4 ℃. v When the temperature of the Temperature-Responsive Cell Culture Surface drops to 4 ℃, and keep it for 20 ~ 30 minutes, then aspirate the culture medium above the Temperature-Responsive Cell Culture Surface with a straw ( cell culture dish), pipette or electric pipette (600ml Cell Culture Flask), and blow the cells attached to the culture surface to make them fall off. During the blowing process, the cell sheet can be observed falling off the Temperature-Responsive surface . v The Temperature-Responsive shedding ability of cells depends on the type of cells, and some cells with strong adherence may be difficult to fall off, which requires multiple blows to fall off. (for example, those digested at 37 ℃ for more than 3 minutes with 0.25% fresh pancreatin are deemed as cells with strong adherence ability).
Storage and Transportation v This product should not be exposed to direct sunlight or exposure for a long time, but can be stored and transported at room temperature.
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